Elevated mitochondrial protein import in acute myeloid leukemia increases reliance on mitochondrial protease LONP1
Most mitochondrial proteins are nuclear encoded, translated in the cytosol, and imported into the mitochondria. Through gene expression analysis and functional assays, we demonstrated that mitochondrial protein import is increased in acute myeloid leukemia (AML) cells compared to normal hematopoietic cells. Increased mitochondrial protein import was positively correlated with increased mitochondrial unfolded protein response (UPRmt), a stress activated pathway of mitochondrial proteases and chaperones that maintains protein solubility and prevents the formation of toxic aggregates. The UPRmt protease LONP1 (Lon Peptidase 1) was upregulated in AML and positively correlated with increased mitochondrial protein import and UPRmt. Genetically or chemically inhibiting the LONP1 ATPase domain induced mitochondrial protein aggregation and selectively killed AML cells with high LONP1 expression while sparing AML cells with low LONP1 expression and normal hematopoietic cells in vitro and in vivo. Thus, we uncovered a critical role of the UPRmt protease LONP1 in buffering stress from mitochondrial protein import in AML.
Authors
- Chaitra Sarathy (ORCID: https://orcid.org/0000-0003-1115-4323)
- Véronique Voisin (ORCID: https://orcid.org/0000-0002-3405-9532)
- Shahbaz Khan (ORCID: https://orcid.org/0000-0001-9352-0088)
- Matthew Tcheng (ORCID: https://orcid.org/0000-0001-5440-6434)
- Yulia Jitkova (ORCID: https://orcid.org/0000-0003-1252-0604)
- Rose Hurren
- Lan-Xin Zhang (ORCID: https://orcid.org/0000-0002-5918-8082)
- Geethu Emily Thomas (ORCID: https://orcid.org/0000-0002-8737-1394)
- Ross S. Mancini (ORCID: https://orcid.org/0009-0004-8821-2565)
- Thomas Kislinger (ORCID: https://orcid.org/0000-0003-3525-5540)
- Edward Ayoub (ORCID: https://orcid.org/0000-0003-1321-0114)
- Nathan Duong (ORCID: https://orcid.org/0000-0001-7171-5554)
- Po Yee Mak
- Siavash Vahidi (ORCID: https://orcid.org/0000-0001-8637-3710)
- Marcela Gronda
- Dakai Ling
- Andrea Arruda (ORCID: https://orcid.org/0000-0003-2516-8005)
- Yongran Yan (ORCID: https://orcid.org/0009-0005-7337-4331)
- Ali Chegini
- Feng Yue
- Bing Z. Carter
- Michael Andreeff
- S M Kornblau
- Zaynab Mamai
- Brady Stock
- Anastasija A. Piric
- Aaron D. Schimmer (ORCID: https://orcid.org/0000-0003-4023-3899)
- Mark D. Minden
- Stefan Quinn W. Currie
- Mark Reed
Institutions
- University Health Network (CA)
- The University of Texas MD Anderson Cancer Center (US)
- Princess Margaret Cancer Centre (CA)
- Ontario Brain Institute (CA)
- University of Guelph (CA)
Publication Details
- Journal
- Journal of Clinical Investigation
- Published
- 2026-06-16
- DOI
- https://doi.org/10.1172/jci196687
- Primary Topic
- Mitochondrial Function and Pathology
- Type
- article
- Field-Weighted Citation Impact
- 0.00