Vibration-Treated Water Alter Metabolic Activity of Primary Mouse Peritoneal Macrophages and RAW 264.7 Cells

It has recently been shown that by sequential vibrational processing (named ‘crossing’) of two closely located vials with the initial substance in one of them and water or lactose in the other, it is possible to obtain novel products, or vibrational iterations. Vibrational iterations are, in certain cases, capable of exerting a modifying effect on the initial substance or its target, thereby altering its physicochemical and biological properties. Since water is the environment in which metabolic processes occur in cells, organs, and tissues, vibrational iterations obtained using water as initial substance can potentially affect their course. In this work, we assessed the effect of vibrational iterations of water on the metabolic activity of primary peritoneal macrophages of C57BL/6 mice and macrophage-like tumor cells of the RAW 264.7 line. It was shown that one vibrational iteration of water increased the metabolic activity of peritoneal macrophages and suppressed the metabolic activity of tumor cells. At the same time, another vibrational iteration of water did not influence the metabolic activity of peritoneal macrophages but decreased the metabolic activity of tumor cells. Probably, taking into account the modifying activity of vibrational iterations, their action is aimed at changing the properties (possibly, structuring) of intracellular water. The results obtained by us show the possibility of a fundamentally new differential effect on the metabolic activity of healthy and tumor cells, which opens up new prospects for antitumor therapy.