Modulatory role of fucoidan against monosodium glutamate-induced reproductive and hepatorenal toxicity in adult male rats

The present study aims to analyse the curative efficacy of fucoidan on MSG mediated changes in hepatorenal and reproductive organs.For this, 42 adult male rats were grouped into six (N = 7animals/group), and treated for 30 consecutive days with following dose regime: Control (saline water), MSG (60 mg/kg), Fucoidan-low-dose (FLD 100 mg/kg), Fucoidan-high-dose (FHD 200 mg/kg), MSG+FLD (60 mg/kg + 100mg/kg) and MSG+FHD (60 mg/kg + 200mg/kg). On day 31, animals were sacrificed, organs were collected, and subjected to sperm parameters, antioxidant enzyme activities, DNA damage and histomorphometric analysis.The findings showed that MSG caused significant decline in sperm parameters and antioxidant enzymes activity, while increasing the concentrations of oxidative stress markers. MSG administration also showed significant DNA damage in sperm, liver and kidney cells. Additionally, adverse histomorphometric changes including damage to seminiferous tubules, caput and caudal epididymal ducts were noted with decline in sperm concentration. Moreover, hepatorenal histological alterations were identified by leukocytic infiltration, cytoplasmic vacuolation in liver and shrinkage of glomerulus and tubular atrophy in the kidney. On contrary, fucoidan treatment reversed all MSG instigated alterations in dose dependent manner.These findings revealed that fucoidan mitigated MSG induced oxidative, genotoxic and histological alterations in rats, suggesting further mechanistic validation for translational relevance.