COMPREHENSIVE ANALYTICAL PROFILING AND STANDARDIZATION OF BILWADI AGAD USING PHYSICOCHEMICAL, MICROBIAL, AFLATOXIN, AND HPTLC METHODS

Bilwadi Agad is a classical polyherbal formulation described inclassical samhitas for the management of various toxic andsystemic conditions. Considering its wide therapeutic utility,scientific standardization is essential to ensure identity, purity,safety, and quality consistency. The present study aimed todevelop a systematic analytical profile of Bilwadi Agad usingmodern evaluation techniques. Materials and Methods:Organoleptic characteristics were assessed through sensoryparameters such as appearance, colour, taste, and odour.Physicochemical evaluation included determination of moisturecontent, ash values, extractive values, and pH followingstandard procedures. Powder microscopy was performed toobserve characteristic diagnostic features. Safety evaluationcomprised aflatoxin estimation and assessment of totalmicrobial load. Chromatographic characterization was carriedout using High-Performance Thin Layer Chromatography(HPTLC) to establish a distinctive fingerprint pattern. Results:Organoleptic evaluation confirmed acceptable sensory characteristics. Microscopic examination revealed diagnostic features indicating authenticity of ingredients. Physicochemical parameters were found within permissible limits. Microbial load and aflatoxin levels complied with safety standards. HPTLC profiling exhibited distinct peaks and Rf values, indicating the presence of multiple phytoconstituents and a characteristic fingerprint profile. Conclusion: The analytical findings affirm the quality, safety, and consistency of Bilwadi Agad. The generated data may serve as reference standards for routine quality control and further standardization of the formulation.