Coating-Free Methods for Forming Liposomes Using Hydrophilic/Hydrophobic Composite Microfluidic Device

We report a simplified strategy for fabricating a device that enables the formation of liposomes in microchannels. The device was prepared by directly patterning hydrophilic and hydrophobic polydimethylsiloxane on the substrate of a mold. After optimization of the fabrication process, we evaluated liposome formation with the resulting device. We observed the solution collected from the outlet by fluorescence microscopy and confirmed liposome formation by identifying particles that encapsulated a fluorescent dye and were surrounded by lipid bilayers. The collected liposomes exhibited good size uniformity and with a coefficient of variation of 13%.