Storage Buffer Composition Impacts Internal Structure, Freeze–Thaw Stability, and Transfection Efficiency of mRNA-Lipid Nanoparticles
phase formation. These findings establish a direct structural link between buffer conditions, particle size, internal morphology, and transfection efficiency, highlighting the importance of buffer composition in modulating mRNA-LNP performance.
Authors
- Mohammed Kawelah (ORCID: https://orcid.org/0009-0003-0462-9383)
- Daniel A. Estabrook (ORCID: https://orcid.org/0000-0001-7237-6291)
- Qiang Cui (ORCID: https://orcid.org/0000-0001-6214-5211)
- Ken K. Qian (ORCID: https://orcid.org/0000-0002-1942-1943)
- Alexander E. Marras (ORCID: https://orcid.org/0000-0001-8972-9532)
- Jiale Shi (ORCID: https://orcid.org/0000-0002-5447-3925)
- Kenneth R. Rodriguez
- Meysam Mohammadi Zerankeshi (ORCID: https://orcid.org/0000-0003-0851-2644)
- Geoffrey T. Nash (ORCID: https://orcid.org/0000-0002-1470-2279)
- Khaled AboulFotouh (ORCID: https://orcid.org/0000-0002-2554-5483)
- Keith P. Johnston (ORCID: https://orcid.org/0000-0002-0915-1337)
- Keira Donnelly
- Noah A. Wambolt
- Dylan J. Charland
- Julia Ennis
- Dipak N. Patil
- Younghoon Oh
- Haocheng Chueh
- Dennis Yang
- Sonia Corba
Institutions
- Seat (Spain) (ES)
- Eli Lilly (United States) (US)
- Professional Solutions (United States) (US)
- Walker (United States) (US)
- The University of Texas at Austin (US)
Publication Details
- Journal
- ACS Nano
- Published
- 2026-06-12
- DOI
- https://doi.org/10.1021/acsnano.5c22170
- Primary Topic
- RNA Interference and Gene Delivery
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Eli Lilly and Company
- Cockrell School of Engineering, University of Texas at Austin
- National Institute of General Medical Sciences