Eukaryotic MAGs from the NEREA observatory: expanding the coastal microbiome dataset
Marine ecosystems are hotspots of biodiversity and biogeochemical activity, yet much of their complexity remains largely inaccessible without genome-resolved data. Here we present a curated dataset of 52 eukaryotic metagenome-assembled genomes (MAGs) reconstructed from samples collected between April 2019 and January 2020 at three NEREA (Naples Ecological REsearch for Augmented observatories) sites in the Gulf of Naples. NEREA is a coastal observatory integrating physical, chemical and biological measurements with state-of-the-art metagenomics. The eukaryotic MAGs have an average completeness of ~55% and genome size of ~20 Mb. Predicted proteins were functionally annotated against UniProtKB, InterPro, and eggNOG databases, and each MAG was taxonomically classified using a curated RNA polymerase A reference dataset. The recovered MAGs encompass diverse eukaryotic lineages, primarily Ochrophyta, Chlorophyta and Haptophyta. Building on the Tara Oceans eukaryotic MAG legacy, this release represents the first reconstruction of eukaryotic MAGs from a coastal time series, enabling temporal and functional analyses of eukaryotic plankton.
Authors
- Bruno Hay Mele (ORCID: https://orcid.org/0000-0001-5579-183X)
- Domenico D’Alelio (ORCID: https://orcid.org/0000-0002-2189-503X)
- Fabio Conversano (ORCID: https://orcid.org/0000-0003-2037-2277)
- Marina Montresor (ORCID: https://orcid.org/0000-0002-2475-1787)
- Luca Ambrosino (ORCID: https://orcid.org/0000-0003-1756-9538)
- Francesca Margiotta (ORCID: https://orcid.org/0000-0003-0757-5934)
- Lucia Campese (ORCID: https://orcid.org/0000-0002-3543-738X)
- Marco Miralto (ORCID: https://orcid.org/0000-0002-4106-4110)
- Raffaella Casotti (ORCID: https://orcid.org/0000-0002-9876-4601)
- Antonio Longo (ORCID: https://orcid.org/0000-0002-9525-1800)
- Eric Pelletier
- Tom O. Delmont
- Karine Labadie
- Pedro Gabriel Barroso de Oliveira (ORCID: https://orcid.org/0009-0002-0452-2511)
- NEREA coordinators
- Patrick Wincker
- Aude Perdereau
- Daniele Iudicone
- Adriana Alberti
Institutions
- Centre National de la Recherche Scientifique (FR)
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
- Université Paris-Saclay (FR)
- Stazione Zoologica Anton Dohrn (IT)
- San Raffaele University of Rome (IT)
- Institut de Biologie Intégrative de la Cellule (FR)
- Genoscope (FR)
- CEA Paris-Saclay (FR)
- Institute of Genetics and Biophysics (IT)
- University of Naples Federico II (IT)
Publication Details
- Journal
- Scientific Data
- Published
- 2026-06-09
- DOI
- https://doi.org/10.1038/s41597-026-07571-y
- Primary Topic
- Genomics and Phylogenetic Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00