Label-Free and In Situ Glycophenotyping Using a Glycocalyx-Mimetic Electrochemical Transducer

-glycosylated mucin domains present tumor-associated truncated O-glycans that serve as linkers to the lectins, our recognition elements. Embedding six lectins within lubricin enables orthogonal recognition of Gal-GalNAc-terminated oligosaccharides. In an electrochemical culture-plate format, the resulting sensor supports real-time, in situ glycophenotyping of melanoma cells, achieving quantitative agreement to a conventional, end point, lectin microarray. This glycocalyx-mimetic transducer thus enables the rapid, label-free screening of lectin-glycan interactions and the profiling of diagnostically relevant, tumor-associated glycans.

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Publication Details

Journal
ACS Applied Materials & Interfaces
Published
2026-06-09
DOI
https://doi.org/10.1021/acsami.6c04290
Primary Topic
Glycosylation and Glycoproteins Research
Type
article
Field-Weighted Citation Impact
0.00

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article

Label-Free and In Situ Glycophenotyping Using a Glycocalyx-Mimetic Electrochemical Transducer

Jessica Da Gama Duarte, Ronil J. Rath, Andreas Behren, Alexandre Xavier Mendes et al.
ACS Applied Materials & Interfaces
Glycosylation and Glycoproteins Research
article

Label-Free and In Situ Glycophenotyping Using a Glycocalyx-Mimetic Electrochemical Transducer

Jessica Da Gama Duarte, Ronil J. Rath, Andreas Behren, Alexandre Xavier Mendes, Luiza Aguiar do Nascimento, Vatsala Pithaih, Henry F. F. Bellette, George Wren Greene, Saimon M. Silva, Simon E. Moulton, Thiago Coimbra Pimenta, Dênio E. Pires Souto, Eliza Grills
article en

Abstract

-glycosylated mucin domains present tumor-associated truncated O-glycans that serve as linkers to the lectins, our recognition elements. Embedding six lectins within lubricin enables orthogonal recognition of Gal-GalNAc-terminated oligosaccharides. In an electrochemical culture-plate format, the resulting sensor supports real-time, in situ glycophenotyping of melanoma cells, achieving quantitative agreement to a conventional, end point, lectin microarray. This glycocalyx-mimetic transducer thus enables the rapid, label-free screening of lectin-glycan interactions and the profiling of diagnostically relevant, tumor-associated glycans.

ACS Applied Materials & Interfaces
La Trobe University (AU), St Vincent's Hospital Melbourne (AU), Biosensores (Spain) (ES), Centro de Espectrometria de Massas Aplicada (Brazil) (BR), Olivia Newton-John Cancer Wellness & Research Centre (AU), Monash University (AU), Swinburne University of Technology (AU)
Australian Research Council
Openalex Percentile: Top 10%
Glycosylation and Glycoproteins Research
DOISourceOpenAlex
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Label-Free and In Situ Glycophenotyping Using a Glycocalyx-Mimetic Electrochemical Transducer — Jessica Da Gama Duarte, Ronil J. Rath, et al. · ACS Applied Materials & Interfaces (2026) | TGRS Research Map | TGRS