ANALYTICAL MEHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF CILNIDIPINE AND METOPROLOL BY UHPLC IN SOLID DOSAGE FORMS

AbstractLiterature review reveals that there is no analytical method reported for the analysis of Cilnidipine andMetoprolol by UHPLC in solid dosage form. Spectrophotometer, HPLC and RP-HPLC are the reportedanalytical methods for compounds either individually or in combination with other dosage form.Hence, it was felt that, there is a need of new analytical method development for the simultaneousestimation of Cilnidipine and Metoprolol by UHPLC in pharmaceutical dosage form. Present work isaimed to develop a new, simple, fast, rapid, accurate, efficient and reproducible Analytical mehoddevelopment and validation for the simultaneous estimation of cilnidipine and metoprolol by UHPLCin solid dosage forms. The developed method will be validated according to ICH guidelines. A simple,Accurate, precise method was developed for the simultaneous estimation of the Cilnidipine andMetoprolol in solid dosage form. Retention time of Cilnidipine and Metoprolol were found 0.61minand 1.20 min respectively. System precision. %RSD of the Cilnidipine and Metoprolol were found0.89 and 1.01 respectively. %Assay were obtained as 99.86% for Cilnidipine and 99.39 % forMetoprolol. Regression equation of Cilnidipine is y = 4.4172x + 1.976. Regression equation ofMetoprolol is y=64.541x+ 79.588. Retention times were decreased and that run time was decreased,so the method developed was simple and economical that can be adopted in regular Quality controltest in Industries.