Nano fountain pen patterning of microtubule tracks for guiding molecular motors

This study explores a new method for transporting biological cargo using motor proteins along their natural tracks, microtubules. Utilizing the nano fountain pen (NFP), we aim to fabricate microtubule pathways connecting designated origin and destination points, offering tracks for kinesin molecules to transport biological cargo. To achieve this, we patterned avidin lines, to which biotinylated microtubules are adhered. The functionality of these immobilized microtubules is confirmed by observing the progressive movement of single kinesin molecules. Furthermore, we demonstrate microtubule alignment through hydrodynamic drag. This method serves as a foundational step toward developing a conveyor network for miniaturized ‘lab-on-a-chip’ systems, with continued potential for advancements in MT alignment and polarization techniques.