Development and Evaluation of Natural Hydrogel Nanocomposites for Skin Rejuvenation: Design and Optimization of Effective Formulations

ABSTRACT Background This study examines the development of innovative hydrogel nanocomposites from natural, biocompatible materials to rejuvenate skin and slow aging. Aim To develop two distinct formulations to counteract internal and external aging factors. Methods The first formulation comprises hyaluronic acid, starch, ascorbic acid, and selenium‐enriched microalgae, with chitosan and polyethylene glycol added. The second formulation combines chitosan, collagen, and Origanum vulgare extract, which are known for their restorative and anti‐aging properties. Research assessed polymer concentration, temperature, pH, and antibacterial properties, optimizing conditions using the RSM model. Results High moisture absorption (85% and 75%) and strong antibacterial activity against gram‐positive bacteria (90% and 85%). Advanced statistical models confirmed the chemical structure and biocompatibility of the nanocomposites. The first formulation exhibited a particle size of about 148 nm, high moisture absorption, and significant antibacterial activity against Staphylococcus aureus . Conclusion Both systems were validated through advanced analyses, demonstrating their efficacy for dermatological applications. The formulations were successfully incorporated into a semisolid cream with an oil‐in‐water emulsion base and exhibit promising physicochemical properties. This research highlights the potential of these nanocomposites for clinical applications in skin rejuvenation products. Future studies are encouraged to explore their use in other topical formulations, such as serums, lotions, and sunscreens.