Bio-supported of silver nanoparticles over chitosan-hyaluronic acidcoated magnetic nanoparticles: a dual-function platform for catalytic organic transformations followed by treatment of pneumonia in mice

Recently, NPs find significant recognition in material science, due to their miniaturized dimension and remarkable physicochemical, and biological properties and has been explicated by increasing number of catalytic and biomedical applications. A novel bio-nanocomposite comprising glutaraldehyde crosslinked and chitosan-hyaluronic acid encapsulated silver nanoparticles supported ferrite is demonstrated herein. Advanced methods including elemental mapping, TEM, FE-SEM, UV-Vis, FT-IR, TEM, EDX, and FE-SEM were used to justify the Fe3O4@CS-HA/Ag NPs nanocomposite. Its catalytic performance was evaluated in A3 coupling reaction for the synthesis of propargylamines as well as the reduction of different nitroarenes under spectroscopic monitoring. The catalyst reusability assay showed that the nanocatalyst was effective for seven consecutive cycles without noticeable diminishing activity. The in vivo research includes analyzing the illness symptoms and assessing the Pseudomonas aeruginosa fatal dose in mice. Calculated lethal dosages, histological analyses, and bacteremia evaluations were used to examine the Fe3O4@CS-HA/Ag NPs effectiveness in treating pneumonia. After 2 days, the untreated animals’ body temperature significantly dropped to 34.7 °C from the original reading of 38.5 °C. By the study end, a 15% decrease in weight was also reported. This survey involves tracking decreases in body weight, hypothermia, and bacteremia. The histological examination demonstrated a sporadic and widespread alveolar gaps inflammatory cells build-up; infiltrates were found in every untreated animal’s lung segment. The goal of this study is to investigate the Fe3O4@CS-HA/Ag NPs biological characteristics to provide a strong remedy for this dangerous infection.