Formulation and Evaluation of Moringa oleifera Leaf Extract Loaded Transdermal Patches with Antioxidant Activity for Rheumatoid Arthritis Management

ABSTRACT Introduction: TDDS are among the best ways to administer medications. through skin into blood circulation, with the advantages of fewer adverse drug effects in the gastrointestinal tract, improved bioavailability, sustained release and avoiding first-pass metabolism. Moringa oleifera is indigenous to the Indian subcontinent, having intense antioxidant and anti-inflammatory activities, studied for thousands of years in traditional medicine, and possessing high bitter and spicy compounds, including flavonoids, polyphenols, vitamins and minerals. It is being studied for RA (rheumatoid arthritis) treatment, which is an aggressive immune illness that progresses over time due to the destruction of the joints. Aim and Objective: To develop and evaluate the M. oleifera leaf extract-loaded transdermal patches and compare the physicochemical and antioxidant activities for RA management. Materials and Methods: M. oleifera leaves were collected, washed, shade-dried and powdered. The extracts were ready for aqueous extraction. The solution-used casting procedure was used to make a drug that used the film-forming polymer like HPMC K, glycerine gelatine as a secondary polymer and dimethyl sulfoxide (DMSO) as the permeation enhancer. Physiological properties have been assessed for solutions. And also various tests are carried out, like moisture absorption and pH, and the technique has been employed to determine antioxidant activity. Results: The prepared patches were smooth, flexible and uniform in appearance. All the formulations showed acceptable physicochemical properties. F3 showed the best antioxidant activity with an IC50 value of 62.2 ppm, followed by F2 (67.5 ppm) and F1 (72.5 ppm), indicating better free detoxification of charges. Conclusion: To possess the ability to select a formulation for the prevention of RA, an M. oleifera transdermal patch was successfully prepared; F3 had the best antioxidant activity. KEY WORDS Transdermal Drug Delivery System (TDDS), M. oleifera, Rheumatoid Arthritis, Antioxidant Activity, Herbal Transdermal Patch.