Deep-UV Resonance Raman Spectroscopy Enables Direct Quantification of mRNA in Intact Lipid Nanoparticle Formulations
Deep-UV resonance Raman spectroscopy (DUV-RRS) enables rapid, label-free quantification of mRNA directly in intact lipid nanoparticles. Without deformulation, seconds-scale acquisitions isolate adenine and guanine signatures with minimal lipid/water interference, yielding calibrated concentration estimates across buffers and formulations. A multipixel SNR strategy improves detection at low signal. This in situ assay offers a high-throughput readout for manufacturing and quality control of mRNA formulations.
Authors
- Ziad Ganim (ORCID: https://orcid.org/0000-0002-8275-269X)
- R. Bhartia (ORCID: https://orcid.org/0000-0002-1434-7481)
- Kathryn Wright
- Angie Kim
- Ray Reid
- Mohindra Seepersaud
- Jamie Gilmore
- Russell Umstead
- Jake Dexter
- Kathleen Korson Sullivan
Institutions
- Modern Electron (United States) (US)
- Moderna Therapeutics (United States) (US)
- Photon Systems (United States) (US)
- Kindeva Drug Delivery (United States) (US)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2026-05-27
- DOI
- https://doi.org/10.1021/acs.analchem.6c02624
- Primary Topic
- Spectroscopy Techniques in Biomedical and Chemical Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00