Taxonomic description of a novel genus, Parajatrophihabitans gen. nov., in the family Jatrophihabitantaceae

Strain DSM 45814 T , isolated from forest soil in Canada, was subjected to a polyphasic taxonomic study and genome mining for plant growth-promoting genes. The strain had a 16S rRNA gene sequence similarity of 97.1% with that of Jatrophihabitans telluris N237 T and 94.8–96.8% similarity with other validly named Jatrophihabitans species. Average nucleotide identity and digital DNA–DNA hybridization values between DSM 45814 T and its closely related Jatrophihabitans strains were below the established prokaryotic species demarcation. In the genome-based phylogeny, strain 45814 T was divergent from the Jatrophihabitans cluster and was loosely associated with the family Geodermatophilaceae . The average amino acid identity (AAI) values between DSM 45814 T and type (63.5–71.6%) and non-type (63.0–71.6%) strains of Jatrophihabitantaceae , as well as members of Geodermatophilaceae (58.6–59.2%), fell within the defined AAI range of 65–72% and below the recently established cut-off point of 74–76% for genus demarcation. The percentage of conserved proteins (POCP) between strain DSM 45814 T and the type strains of Jatrophihabitantaceae and Geodermatophilaceae was below the defined threshold of 50% for genus demarcation, excluding J. telluris , which had a POCP of 55%. Strain DSM 45814 T displayed cocci-to-cuboid cells with a flagellum and a G+C content (63.4 mol%) that distinguished it from Jatrophihabitans and Geodermatophilaceae strains, but it had chemotaxonomic features closer to those of Jatrophihabitantaceae than Geodermatophilaceae . The strain appeared to have ecological potential based on a genome mining approach. Based on these results, strain DSM 45814 T (=899 T =LMG 34134 T ) represents a novel genus within the family Jatrophihabitantaceae , for which the name Parajatrophihabitans canadensis gen. nov. sp. nov. is proposed.