AVITHRAPID - Standard Operating Procedure - Antiviral activity evaluation by qPCR – Virucidal compounds
When evaluating the antiviral effect of a compound, assessing cellular toxicity in the target cell line is an essential prerequisite (see dedicated SOP). The highest dose allowing > 90 % cell viability is the first tested dose in antiviral assay. Quantifying viruses in the supernatants of infected cell cultures enables the evaluation of the antiviral efficacy of compounds. Vero E6 cell line is used for SARS-CoV-2 infection and Huh7 cell line for WNV and CHIKV infection.
Authors
- Anne Bull-Maurer
Publication Details
- Journal
- Zenodo (CERN European Organization for Nuclear Research)
- Published
- 2026-05-27
- DOI
- https://doi.org/10.5281/zenodo.20408330
- Primary Topic
- SARS-CoV-2 and COVID-19 Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00