Two-Photon Microscopy in Biological Imaging: Current Uses and Evolving Potential
Two-photon microscopy (TPM) has become an indispensable tool in the life sciences, offering exceptional spatial resolution, deep tissue penetration, and low phototoxicity. It provides a revolutionary solution for long-term dynamic observation of living cells and tissues. This article systematically explains the working principles of TPM and reviews its applications in cutting-edge fields such as neuroscience, immunology, oncology, regenerative science and plant biology, highlighting its remarkable multidisciplinary adaptability. Building on this foundation, we further explore the breakthrough directions and future prospects for the development of this technology.
Authors
- Shiwei Yuan (ORCID: https://orcid.org/0000-0002-6754-784X)
- Xingyun Zhang (ORCID: https://orcid.org/0000-0002-6658-9319)
- Kai Song (ORCID: https://orcid.org/0000-0001-6792-7159)
- Yinghui Gu
- Xiaodan Wang
- Qinpan Bu
Institutions
- Chinese Academy of Sciences (CN)
- Changchun Normal University (CN)
- Changchun Institute of Optics, Fine Mechanics and Physics (CN)
Publication Details
- Journal
- Photonics
- Published
- 2026-05-27
- DOI
- https://doi.org/10.3390/photonics13060519
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Changchun Normal University