Sodium alginate functionalized biocompatible selenium nanocarriers loaded trans-resveratrol for mitochondrial targeted HepG2 hepatocellular carcinoma inhibition study

AIMS: To investigate the use of sodium alginate (SA) for functionalization of selenium nanoparticles (SA@Se NPs) in developing biocompatible nanocarrier to enhance stability, mitochondrial targeting and anticancer efficacy of trans-resveratrol (TRV). METHODS: SA@Se NPs were synthesized by chemical reduction method and characterized to evaluate their crystalline structure by X-ray diffraction (XRD), particle size and surface charge. Synthesized SA@Se NPs were evaluated for cumulative TRV release. Cytotoxicity of TRV-SA@Se was assessed against HepG2 and HEK-293T cells by WST-8 assay. Superoxide anion detecton by staining with MitoSOX and calorimmetrically caspases-9/3 activities were evaluated in HepG2 cells. RESULTS: of 35 µg/mL while maintained higher viability against normal cells. TRV-SA@Se NPs significantly stimulated intercellular ROS and mitochondrial superoxide level with caspases-9/3 activities. CONCLUSION: , SA@Se NPs demonstrated pH-responsive TRV release and increase mitochondrial targeted anticancer activity against HepG2 cells.