Expression of Genes Associated with Epithelial to Mesenchymal Transition in MCF-7 Breast Cancer Cells Treated with Monocarbonyl Analogs of Curcumin C66 and B2BrBC—RT-qPCR Array Dataset

Curcumin is a polyphenolic bio-compound derived from the rhizomes of the turmeric plant (Curcuma longa) that has proven anti-carcinogenic properties but poor bioavailability. By modifying its chemical structure, the monocarbonyl analogs of curcumin (MACs) possess improved stability, resorption, and circulation. This dataset presents RT-qPCR array analysis of 84 genes associated with Epithelial to Mesenchymal Transition (EMT), a key early event in cancer progression and metastasis, in human MCF-7 breast cancer cells. Cells were stimulated toward EMT reprogramming by treatment with a combination of EMT-inducing factors and co-treated with two experimental MACs, C66 or B2BrBC. Gene expression was measured using the human EMT QIAGEN RT2 Profiler kit, and results were obtained from three independent experiments. Gene expression changes are presented as both fold regulation and fold change values, with statistical significance determined by Student’s t-test (p < 0.05). This comprehensive dataset enables investigation into how MACs modulate the EMT transcriptome in breast cancer cells, with potential applications for understanding EMT mechanisms. The raw and processed data are publicly available and can be used for comparative analyses, validation studies, and bioinformatic analyses of EMT-related signaling pathways.