delimtools: an R package for species delimitation

Single-locus species-delimitation methods using DNA barcoding data have made an important contribution to large scale biodiversity inventories and integrative taxonomy, allowing rapid and repeatable estimates of alpha diversity from huge numbers of individuals. A range of distance-based and tree-based methods now exist to perform these analyses, including ABGD, ASAP, GMYC, and mPTP. However, the outputs of such software are idiosyncratic and difficult to integrate and compare. The R package delimtools offers a platform capable of: (1) pre-processing, cleaning and formatting data; (2) collapsing and summarising haplotype information; (3) executing, parsing and merging program outputs and species partitions; and (4) tabulating results, and visualizing on a phylogenetic tree. The open-source delimtools package opens up new avenues to scale up and standardize species delimitation analyses, and is available on the CRAN (https://cran.r-project.org/package=delimtools) and GitHub (https://github.com/legalLab/delimtools/) repositories.

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Publication Details

Journal
Genome
Published
2026-05-19
DOI
https://doi.org/10.1139/gen-2025-0119
Primary Topic
Genetic diversity and population structure
Type
article
Field-Weighted Citation Impact
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article

delimtools: an R package for species delimitation

Pedro Bittencourt, R. A. Collins, Tomas Hrbek
Genome
Genetic diversity and population structure
article

delimtools: an R package for species delimitation

Pedro Bittencourt, R. A. Collins, Tomas Hrbek
article en

Abstract

Single-locus species-delimitation methods using DNA barcoding data have made an important contribution to large scale biodiversity inventories and integrative taxonomy, allowing rapid and repeatable estimates of alpha diversity from huge numbers of individuals. A range of distance-based and tree-based methods now exist to perform these analyses, including ABGD, ASAP, GMYC, and mPTP. However, the outputs of such software are idiosyncratic and difficult to integrate and compare. The R package delimtools offers a platform capable of: (1) pre-processing, cleaning and formatting data; (2) collapsing and summarising haplotype information; (3) executing, parsing and merging program outputs and species partitions; and (4) tabulating results, and visualizing on a phylogenetic tree. The open-source delimtools package opens up new avenues to scale up and standardize species delimitation analyses, and is available on the CRAN (https://cran.r-project.org/package=delimtools) and GitHub (https://github.com/legalLab/delimtools/) repositories.

Genome
Brazilian Institute of Environment and Renewable Natural Resources (BR), Natural History Museum (GB), Trinity University (US), Universidade Federal do Amazonas (BR)
Life in Land
Openalex Percentile: Top 4%
Genetic diversity and population structure
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