Triiodothyronine-driven pro-inflammatory responses of dendritic cells are restrained by sphingolipid signaling

We previously reported that triiodothyronine (T3) promotes dendritic cell (DC) maturation and enhances their ability to induce pro-inflammatory and cytotoxic T-cell responses through Akt signaling. However, the underlying mechanisms remain incompletely understood. Sphingosine-1-phosphate (S1P), a bioactive sphingolipid, is implicated in several pro-inflammatory conditions. Here, we investigated the role of sphingosine kinase 1 (SK1), S1P, and its receptors (S1PRs) in the immunomodulatory effects of T3 on DCs and the ensuing adaptive immune response. DCs were generated from bone marrow of C57BL/6 wild-type or SK1 knockout mice and stimulated with T3 (T3-DC). To modulate the S1P pathway, PF-543 (SK1 inhibitor), S1P, or FTY720 (S1PR functional antagonist) were added prior to T3. Phosphorylated Akt (p-Akt) and phosphorylated STAT3 (p-STAT3) were analyzed by Western blot. Splenocytes from BALB/c mice were co-cultured with DCs under SK1 or S1PR inhibition and exposed to T3. Cell markers and proliferation were evaluated by flow cytometry, and cytokines measured by flow cytometry and ELISA. We show that the SK1/S1P/S1PR pathway regulates IL-12p70 production in T3-DC, while S1PRs also modulate IL-6 secretion. Mechanistically, S1P signaling mediates T3-induced Akt phosphorylation in DCs. STAT3 activation was observed in T3-DC and was not altered by inhibition of SK1 or S1PR. Although the SK1/S1P/S1PR axis did not alter T cell proliferation, S1PR inhibition increased IFN-γ, and inhibition of either SK1 or S1PRs enhanced IL-17 secretion by splenocytes. Altogether, these findings suggest that a complex sphingolipid-mediated signaling network modulates the immunostimulatory effects of T3 on DCs and the driven adaptive immunity.