Spatiotemporal Variability of Water Quality Along an Altitudinal Gradient in a Tropical River Basin: The Chiriquí Viejo River (Panama)

This study evaluated spatial and seasonal patterns of physicochemical water quality in the Chiriquí Viejo River basin (western Panama), a tropical watershed characterized by strong seasonal variability. A total of 90 water samples were collected at ten stations during the rainy season (May to October 2024) and dry season (January to March 2025). Dissolved oxygen (DO), turbidity, potential of hydrogen (pH), apparent color, total dissolved solids (TDS), and electrical conductivity (EC) were analyzed following ISO/IEC 17025:2017 accredited methods, and precipitation patterns were characterized using spatial interpolation of meteorological data. Spatio-temporal variability was assessed using linear mixed-effects models, with season and basin position as fixed effects and sampling site as a random factor. Results showed a spatial and seasonal structuring of water quality, with the upper basin exhibiting high and stable DO concentrations and low turbidity and apparent color. In contrast, the middle and lower basin showed rainy-season increases in turbidity and apparent color, supported by a significant season × basin interaction, indicating that precipitation driven impacts are heterogeneous along the basin. EC and TDS displayed spatial gradients, while DO remained relatively stable across seasons and basin levels. These findings highlight turbidity and apparent color as sensitive indicators of precipitation-driven impacts.