CHROMATOGRAPHIC AND COMPUTATIONAL APPROACHES TO THE PHARMACOKINETIC PROFILE OF PIROXICAM AND ITS SENSITIVE ASSAY IN RABBIT PLASMA
Objective: A simple, rapid, sensitive, and cost-effective high-performance liquid chromatographic (HPLC) method was developed for the pharmacokinetic study and determination of Piroxicam.Material and Method: The chromatographic separation was performed with a Agilent ZORBAX reversed-phase C18 column (250 mm × 4,6 mm ID × 5 μm). Mobile phase composition was 0.04 M KH2PO4: acetonitrile:methanol (50:40:10) at pH 3.8. The flow rate was adjusted as 0.9 mL/min.Result and Discussion: An isocratic method was used for the analysis. The UV detector was set at 360 nm, and a retention time of 7 minutes was obtained for piroxicam. The method showed linear performance over the concentration range of 0.1–4 mg/l.
Authors
- Özgür Eşi̇m (ORCID: https://orcid.org/0000-0002-6402-156X)
- Ayhan Savaşer (ORCID: https://orcid.org/0000-0001-8342-2919)
- Yalçın Özkan (ORCID: https://orcid.org/0000-0001-5163-6867)
- Berk Alp Göksel (ORCID: https://orcid.org/0000-0002-1610-0618)
- Engin Koçak (ORCID: https://orcid.org/0000-0002-1076-1300)
- Nurgül K. Bakırhan (ORCID: https://orcid.org/0000-0001-5469-9142)
- Okan Ali Aksoy (ORCID: https://orcid.org/0000-0003-4954-137X)
- Esra Merve Zanbak Çotaoğlu (ORCID: https://orcid.org/0000-0002-6607-8973)
- Tugba Taskın Tok (ORCID: https://orcid.org/0000-0002-0064-8400)
- Sibel A. Özkan (ORCID: https://orcid.org/0000-0001-7494-3077)
Institutions
- Ankara University (TR)
- Akdeniz University (TR)
- Sağlık Bilimleri Üniversitesi (TR)
- University of Health Sciences (SO)
Publication Details
- Journal
- Ankara Universitesi Eczacilik Fakultesi Dergisi
- Published
- 2026-05-14
- DOI
- https://doi.org/10.33483/jfpau.1766558
- Primary Topic
- Analytical Methods in Pharmaceuticals
- Type
- article
- Field-Weighted Citation Impact
- 0.00