Large-Scale Chemical-Genetic Interaction Profiling Identifies a Small-Molecule Inhibitor of <i>Mycobacterium tuberculosis</i> Polyketide Synthase 13
detoxifying enzymes, including Rv2581c. These results highlight how PROSPECT can not only immediately reveal, with domain-level resolution, the MOA of new inhibitors, allowing the integration of biological insight into early prioritization, but can also illuminate genetic interactions linked to those mechanisms that could inform synergy predictions for combination therapy.
Authors
- Austin Bond (ORCID: https://orcid.org/0009-0001-8486-0619)
- Sulyman Barkho
- Diana K. Hunt (ORCID: https://orcid.org/0009-0005-7069-0291)
- Emily J. Geddes (ORCID: https://orcid.org/0000-0002-7756-1792)
- Pooja Chaudhary
- Rebecca J Ulrich
- Deborah T. Hung (ORCID: https://orcid.org/0000-0003-4262-0673)
- Matthew Y. Solomon
- Cong Liu (ORCID: https://orcid.org/0000-0002-1687-2220)
- James E. Gomez (ORCID: https://orcid.org/0000-0002-2640-7453)
Institutions
- Broad Institute (US)
- Harvard University (US)
- Massachusetts General Hospital (US)
- Integrated Solutions for Systems (United States) (US)
Publication Details
- Journal
- ACS Infectious Diseases
- Published
- 2026-05-13
- DOI
- https://doi.org/10.1021/acsinfecdis.6c00136
- Primary Topic
- Tuberculosis Research and Epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Bill and Melinda Gates Foundation