From polyclonal to monoclonal: de novo sequencing of goat antibodies for a standardized ApoA-I immunoturbidimetric assay
= 0.994). This optimized cocktail successfully replaced the pAb reagent, matching or exceeding its performance in key analytical parameters including linearity, sensitivity, and precision. Our work thus establishes a practical pathway for converting heterogeneous pAbs into defined, recombinant antibody reagents suitable for standardized IVD applications.
Authors
- Qitao Song
- Lulai Xu
- Linzhu Li
- Sai Li
- Ming Zhu
- Zhengzhong Song
- Xin Yang
- Bohong Li
- Yun Xie
- Jing Wang (ORCID: https://orcid.org/0009-0003-7060-0589)
- Weijing Yi
- Wei Zhao
- Qiang Li
- Bin Ma
Institutions
- Sichuan University (CN)
- Lianyungang Oriental Hospital (CN)
- Shanghai CASB Biotechnology (China) (CN)
- Northwest Women's and Children's Hospital (CN)
- Chongqing University of Education (CN)
- The First People’s Hospital of Lianyungang (CN)
- Chongqing Municipal Government (CN)
- Sichuan Center for Disease Control and Prevention (CN)
Publication Details
- Journal
- Journal of Biological Engineering
- Published
- 2026-05-12
- DOI
- https://doi.org/10.1186/s13036-026-00697-y
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China