Rational Sequence Modification Upgrades a Food-Derived Peptide into a Stronger Dual-Functional Regulator of α-Glucosidase Activity and Insulin Resistance
= 0.126 ± 0.008 mM) exhibited stronger α-glucosidase inhibition than FAPSW and acarbose. Molecular docking suggested that hydrogen bonds were not the sole determinants of peptide-mediated α-glucosidase inhibition, while molecular dynamics simulations revealed that the enhanced inhibitory activity of YAPSW primarily resulted from its more stable binding to 3A4A. Moreover, FAPSW and YAPSW could improve IR and YAPSW exhibited stronger effects on IRS-1/PI3K/AKT signaling pathway than FAPSW. This study provides insights into optimizing α-glucosidase inhibitory peptides and supports the potential application of YAPSW as a multifunctional food ingredient for T2DM management.
Authors
- Yejun Deng (ORCID: https://orcid.org/0000-0002-4855-9378)
- Yuechao Zhang
- Ying Liu (ORCID: https://orcid.org/0000-0001-8931-9656)
- Junhao Wan
- J. Tian
- Xiang Wang
- Lixin Huang (ORCID: https://orcid.org/0000-0002-8507-1910)
- Pujun Xie (ORCID: https://orcid.org/0000-0003-0534-1851)
- Caihong Zhang
Institutions
- Nanjing Forestry University (CN)
- Dalian Medical University (CN)
- Second Affiliated Hospital of Chongqing Medical University (CN)
- Institute of Chemical Industry of Forest Products (CN)
- Chinese Academy of Forestry (CN)
- State Forestry and Grassland Administration (CN)
- Pharmaceutical Biotechnology (Czechia) (CZ)
- Chongqing Medical University (CN)
- Tsinghua University (CN)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2026-05-12
- DOI
- https://doi.org/10.1021/acs.jafc.6c01142
- Primary Topic
- Regulation of Appetite and Obesity
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China