A rapid yeast-based reverse genetics system reveals SARS-CoV-2 Omicron BA.2.86 variant spreads faster than Omicron JN.1 variant in primary human nasal and bronchial epithelial airway cultures
. This method is easy, convenient, and highly efficient, generating infectious cDNA clones within 2 weeks. This system could readily be adapted to construct infectious cDNA clones for other large RNA viruses.
Authors
- Phylip Chen (ORCID: https://orcid.org/0000-0001-6090-6320)
- Prosper N. Boyaka (ORCID: https://orcid.org/0000-0001-6857-9088)
- E Cormet-Boyaka
- Michelle Chamblee (ORCID: https://orcid.org/0000-0002-9794-4504)
- Xueya Liang
- Cheng Chih Hsu
- Fei Jiang
- Jiayu Xu
- Amal O. Amer
- Mark E. Peeples (ORCID: https://orcid.org/0000-0002-4582-317X)
- Jianrong Li (ORCID: https://orcid.org/0000-0002-7130-1084)
- Yuexiu Zhang
Institutions
- Nationwide Children's Hospital (US)
- The Ohio State University (US)
Publication Details
- Journal
- mBio
- Published
- 2026-05-11
- DOI
- https://doi.org/10.1128/mbio.00122-26
- Primary Topic
- SARS-CoV-2 and COVID-19 Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Cystic Fibrosis Research
- National Institutes of Health