A bioluminescent activity dependent platform, BLADe, for converting intracellular activity to photoreceptor activation
fluctuations via locally generated bioluminescence control neural dynamics through opsin activation in single cells, populations and intact tissue. BLADe can be expanded to couple any signal that bioluminescent enzymes can be engineered to detect with the wide variety of photosensing actuators. This modular strategy of coupling an activity dependent light emitter to a light sensing actuator offers, in principle, a generalizable framework for state dependent cell-autonomous control across biological systems.
Authors
- Zohair Zaidi
- Alexander D. Silvagnoli
- Diane Lipscombe (ORCID: https://orcid.org/0000-0002-7146-9119)
- Mansi Prakash (ORCID: https://orcid.org/0000-0001-7980-413X)
- Akash Pal (ORCID: https://orcid.org/0000-0002-2088-3897)
- Manuel Gomez‐Ramirez (ORCID: https://orcid.org/0000-0002-1017-5256)
- Christopher I. Moore (ORCID: https://orcid.org/0000-0003-4534-1602)
- Maya O. Tree
- Emmanuel L. Crespo
- Ute Hochgeschwender
- Nathan C. Shaner
Institutions
- Allen Institute for Brain Science (US)
- Central Michigan University (US)
- Duke University (US)
- Brown University (US)
- University of Michigan (US)
- University of California San Diego (US)
- Max Planck Florida Institute for Neuroscience (US)
- Division of Undergraduate Education (US)
Publication Details
- Journal
- Scientific Reports
- Published
- 2026-05-11
- DOI
- https://doi.org/10.1038/s41598-026-51072-2
- Primary Topic
- bioluminescence and chemiluminescence research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- W. M. Keck Foundation
- National Institutes of Health
- Division of Chemical, Bioengineering, Environmental, and Transport Systems