Lithocholic Acid-Derived Imidazolium Salts Suppress Breast Cancer Growth: In Vitro and In Vivo Evaluation

PURPOSE: A series of imidazolium salts (IMS), substituted with lithocholic acid (LA) and alkyl chains of varying lengths (LA-1 to LA-10), were evaluated for their cytotoxic effects against breast cancer (BC). METHODS: The cytotoxic potential of the tested salts was assessed in estrogen-dependent (MCF-7) and non-estrogen-dependent (MDA-MB-231) cell lines, as well as in the cardiomyocyte cell line H9C2. The study was also conducted in vivo using a MCF-7 mouse xenograft model. RESULTS: = 51.71 µg/mL). In vivo studies demonstrated that LA-8 treatment significantly inhibited the growth of MCF-7 tumors. CONCLUSIONS: LA-based IMS demonstrate significant potential as chemotherapeutic agents for the treatment of breast cancer.