Extending Serum IgG1 Antibody Repertoire Coverage Using DIA-PTCR
region was found to be sufficient to cover the total Fab distribution. This gas-phase fractionation, when also combined with reduced noise filtering, increased the number of clones detected within a single run from 1043 to 2063 (+100%). Importantly, quantitative data on antibody abundances could be retained as required for repertoire profiling. We conclude that DIA-PTCR is beneficial for LC-MS-based antibody repertoire profiling.
Authors
- Albert Bondt (ORCID: https://orcid.org/0000-0002-0985-7903)
- Kyle L. Fort
- Rien Leuvenink
- Linus Wollenweber (ORCID: https://orcid.org/0009-0008-5256-594X)
- Albert J. R. Heck (ORCID: https://orcid.org/0000-0002-2405-4404)
Institutions
- Utrecht University (NL)
- Thermo Fisher Scientific (Germany) (DE)
Publication Details
- Journal
- Journal of the American Society for Mass Spectrometry
- Published
- 2026-05-11
- DOI
- https://doi.org/10.1021/jasms.6c00051
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek