Biosynthetic Toxin Mimics Meet Nanophotonics: A Rapid, Label-Free Biosensor Platform for Real-Time Detection of Bongkrekic Acid in Urine
of 0.64 ng/mL and a limit of detection of 0.12 ng/mL, representing a 10-fold improvement over the ic-ELISA using conventional conjugates and providing a performance comparable to LC-MS/MS. The method was successfully validated in spiked blind urine samples, highlighting its applicability in clinically relevant matrices. The combination of BA-MBP fusion conjugate with photonic biosensing establishes a robust, safe, and scalable approach for rapid, reliable BA detection and quantification, with strong potential for point-of-care diagnostics and decentralized toxin monitoring.
Authors
- Hui-Jun Fu
- Laura M. Lechuga (ORCID: https://orcid.org/0000-0001-5187-5358)
- M.‐Carmen Estévez (ORCID: https://orcid.org/0000-0003-3694-7186)
- Xue-Ming Cao
- Andrés Alonso-Fernández
- Zhen-Lin Xu (ORCID: https://orcid.org/0000-0002-1197-4615)
- Patricia Ramirez-Priego
Institutions
- South China Agricultural University (CN)
- Xihua University (CN)
- Sichuan University (CN)
- Institut Català de Nanociència i Nanotecnologia (ES)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2026-05-11
- DOI
- https://doi.org/10.1021/acs.analchem.6c01567
- Primary Topic
- Carbon and Quantum Dots Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Ministerio de Ciencia, Innovación y Universidades
- National Natural Science Foundation of China
- China Scholarship Council
- Natural Science Foundation of Sichuan Province
- Departament d'Universitats, Recerca i Societat de la Informació