Mitofusin-Decorated Extracellular Vesicles Enable Targeted Nucleic Acid Delivery to Mitochondria
models, siRNA-loaded MFNEVs effectively reduce the expression of mitochondrial DNA-encoded genes. Moreover, sgRNA-loaded MFNEVs can achieve CRISPR-based mitochondrial gene editing, resulting in a decreased mitochondrial DNA content. Mechanistic studies further reveal that the delivery is facilitated by the cooperation of the mitochondrial fusion machinery. These findings establish the feasibility and versatility of MFNEVs as a promising delivery solution for mitochondrial therapeutics.
Authors
- Jiafeng Zhong (ORCID: https://orcid.org/0009-0007-8988-2909)
- Xing Chang
- Xuehan Xu
- Wenjing Xuan
- Jianjun Cheng (ORCID: https://orcid.org/0000-0003-2561-9291)
- Chengjie Sun (ORCID: https://orcid.org/0000-0001-6038-1616)
- Luyao Wang
Institutions
- Fudan University (CN)
- Westlake University (CN)
- Institute for the Future (US)
- Institute for Advanced Study (DE)
- Indiana University School of Medicine
Publication Details
- Journal
- Nano Letters
- Published
- 2026-05-11
- DOI
- https://doi.org/10.1021/acs.nanolett.6c00233
- Primary Topic
- Mitochondrial Function and Pathology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China