CD177+neutrophils promote endothelial senescence through extracellular traps in Behçet's Disease

OBJECTIVE: PMN dysregulation on endothelial cells in Behçet's disease (BD), a chronic systemic vasculitis characterized by polymorphonuclear neutrophil (PMN) activation and endothelial dysfunction. METHODS: PMN-derived NETs were assessed. RESULTS: PMN-derived NETs contained more Histone 3.1 (H3.1). H3.1 induced senescence, SASP and PMN chemotaxis in VECs via TLR4 signaling, which was attenuated by senolytics. CONCLUSION: PMN in BD patients promote senescence and SASP in VECs through secreting H3.1-enriched NETs, which recruit PMN and collectively contribute to vasculitis in BD.