Engineering of Riboflavin Transporter and Enzymes in <i>Bacillus subtilis</i> Facilitates High-Efficiency FAD Synthesis from RF
-FAD) of 92% in shaking-flask fermentation optimization. This work achieved a 225-fold increase in FAD production, validating the effectiveness of the uptake-catalysis synergistic strategy, thereby establishing an efficient and scalable synthesis paradigm.
Authors
- Yunlong Wu (ORCID: https://orcid.org/0000-0003-1674-3987)
- Xiaole Xia (ORCID: https://orcid.org/0000-0002-9063-9507)
- Yuhao Sun
- Huan Miao
- Ling Gao (ORCID: https://orcid.org/0000-0002-4501-5412)
Institutions
- Jiangnan University (CN)
- Ministry of Education Science and Technology (MW)
Publication Details
- Journal
- ACS Synthetic Biology
- Published
- 2026-05-11
- DOI
- https://doi.org/10.1021/acssynbio.6c00153
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Basic Research Program of Jiangsu Province
- National Key Research and Development Program of China