End-to-End Workflow for 3D Digitization of Biological Specimens Using scAnt and Photogrammetry

This document describes a complete workflow for the 3D digitization of biological specimens using the open-source scAnt scanner and photogrammetric reconstruction in Agisoft Metashape.The guide covers scanner setup, image acquisition, focus stacking, mask generation, post-processing, and 3D reconstruction workflows, including practical recommendations and limitations identified during use. Although primarily designed for arthropods and medium-sized biological specimens, the workflow may be adapted to a wide variety of small objects requiring high-quality 3D digitization.

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Publication Details

Journal
Zenodo (CERN European Organization for Nuclear Research)
Published
2026-05-11
DOI
https://doi.org/10.5281/zenodo.20121539
Primary Topic
Cell Image Analysis Techniques
Type
article
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article

End-to-End Workflow for 3D Digitization of Biological Specimens Using scAnt and Photogrammetry

Sebastián Enrique López-Pérez
Zenodo (CERN European Organization for Nuclear Research)
Cell Image Analysis Techniques
article

End-to-End Workflow for 3D Digitization of Biological Specimens Using scAnt and Photogrammetry

Sebastián Enrique López-Pérez
article en

Abstract

This document describes a complete workflow for the 3D digitization of biological specimens using the open-source scAnt scanner and photogrammetric reconstruction in Agisoft Metashape.The guide covers scanner setup, image acquisition, focus stacking, mask generation, post-processing, and 3D reconstruction workflows, including practical recommendations and limitations identified during use. Although primarily designed for arthropods and medium-sized biological specimens, the workflow may be adapted to a wide variety of small objects requiring high-quality 3D digitization.

Zenodo (CERN European Organization for Nuclear Research)
Openalex Percentile: Top 7%
Cell Image Analysis Techniques
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