Effects of secretome derived from resveratrol treated mesenchymal stem cell on cornea wound: an organ culture study

The aim of this study was to improve of therapeutic effects of Adipose-Derived Stem Cells (ADSCs) secretome with Resveratrol (RV) treatment and investigate its effect on corneal wound healing. The ADSCs were isolated from the abdominal subcutaneous fat tissue of a rat and subsequntly cultured. Epidermal Growth Factor (EGF) and Hepatocyte Growth Factor (HGF) concentration were measured in secretome. Then, the effects of secretome treatment was assessed on rat cornea wound in ex-vivo model. The wound area and thickness of epithelial layer were measured and analyzed. The findings indicated that RV significantly increased the levels of HGF and EGF in the secretome. Furthermore, the secretome derived from RV-treated ADSCs resulted in a reduction of the corneal wound area and increased re-epithelialization. This study shown secretome derived from RV treated ADSCs can be effective for repair of cornea wound. Therefor, the RV treated stem cell approach hold promise to enhance the therapeutic effects of stem cell secretome.