PHARMACOGNOSTICAL ASSESSMENT OF ALLAMANDA BLANCHETII LEAVES

The pharmacognostical study of Allamanda blanchetii leaves revealed key diagnostic features: dorsiventral lamina with uniseriate epidermis and thick cuticle, Anisocytic stomata on the lower epidermis, and uniseriate trichomes on both surfaces. Mesophyll showed 1–2 layers of palisade cells with oil globules and spongy parenchyma with intercellular spaces. The midrib exhibited collenchymatous support and crescent-shaped bicollateral vascular bundles. Powder microscopy identified rosette, cluster, and prismatic crystals, spiral and pitted vessels, and characteristic trichomes. These anatomical markers provide reliable criteria for identification and quality assessment. Physicochemical parameters including Leaf constant such as stomatal number & stomatal index, Vein islet and vein termination, moisture content, total ash, acid‑insoluble ash, water‑soluble ash, sulphated ash, extractive values and fluorescence analysis were determined. These findings establish distinctive markers for the identification and differentiation of Allamanda blanchetii leaves and provide a valuable reference framework for future phytochemical research.