Benchmarking Dendritic Cell Enrichment Methods Reveals Subset‐Specific Recovery Biases
Dendritic cells (DCs) are rare in human blood, often requiring enrichment prior to analysis. Here, we benchmark commonly used pan-DC enrichment methods by comparing recovered DC subset composition to ex vivo references. While all approaches capture major DC populations, recovery is partial and exhibits method-dependent, subset-specific biases. These differences alter the apparent DC composition and may influence downstream analyses. Created with BioRender.com.
Authors
- Lucile Massenet-Regad (ORCID: https://orcid.org/0000-0003-1629-8983)
- Pierre Tonnerre (ORCID: https://orcid.org/0000-0002-1719-9736)
- Emilie Artru
- Fanny Onodi (ORCID: https://orcid.org/0000-0002-8429-4821)
- Diane Biron
- Margaux Bossis
- Vassili Soumelis
- Enwono Eyoh
Institutions
- Inserm (FR)
- Sorbonne Paris Cité (FR)
- Institut Cochin (FR)
- Hôpital Saint-Louis (FR)
Publication Details
- Journal
- Immunology
- Published
- 2026-05-09
- DOI
- https://doi.org/10.1111/imm.70145
- Primary Topic
- Immunotherapy and Immune Responses
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Agence Nationale de la Recherche
- Institut National de la Santé et de la Recherche Médicale
- Fondation pour la Recherche Médicale
- Ligue Contre le Cancer
- H2020 Marie Skłodowska-Curie Actions