Early Detection of Pulmonary Mucormycosis Using Microbial Cell-Free DNA Sequencing of Bronchoalveolar Lavage Fluid in Neutropenic Hosts: A Report of Two Cases

Mucormycosis is an aggressive fungal infection characterized by angioinvasion, tissue necrosis, and high mortality rates. Microbial cell-free DNA (mcfDNA) sequencing provides rapid, non-invasive detection of microbial genetic material to guide diagnosis and treatment. We describe 2 cases of immunocompromised patients with invasive mucormycosis wherein mcfDNA sequencing significantly expedited diagnosis, allowing early surgical intervention and antimicrobial treatment.