Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation

AIM: To investigate the role of hydrogel composition and nanozymes incorporation on the inflammatory response of encapsulated murine microglia. METHODS: -NPs) were prepared and characterized. The inflammatory response of encapsulated microglia was assessed. RESULTS: promoted a slight pro-inflammatory response, with a 1.3-fold and 1.2-fold increase in IL-1β and TNF-α expression. However, prolonged LPS exposure reflected an anti-inflammatory scenario, with increased IL-4 and a 2.4-fold increase in Arg-1 expression. CONCLUSION: SA hydrogels are promising carriers for antioxidant nanozymes and microglia, modulating cell response under prolonged inflammation while protecting cells from oxidative stress.