A high sensitivity strategy to screen NAD(P)H-dependent reductase activity by coupled enzyme cascade
, the coupled enzyme cascade triggers cleavage of a fluorogenically labeled probe, releasing a strong fluorescent signal. This allows detection of very low levels of a specific oxidoreductase activity that one may wish to magnify by directed evolution using our assay in high-throughput screening.
Authors
- David H. Kwan (ORCID: https://orcid.org/0000-0003-2061-7799)
- Jacob Sicheri
- Trisha Ghosh
Institutions
- Université de Sherbrooke (CA)
- Concordia University (CA)
Publication Details
- Journal
- Journal of Biological Chemistry
- Published
- 2026-05-01
- DOI
- https://doi.org/10.1016/j.jbc.2026.113135
- Primary Topic
- Metal-Catalyzed Oxygenation Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Natural Sciences and Engineering Research Council of Canada