Evaluation of <i>Bna.SCT</i> and <i>Bna.REF1</i> as Target Genes to Reduce Sinapine in Rapeseed Using a Protoplast‐Based <scp>CRISPR RNP</scp> Approach
plants with a single sgRNA. Seed sinapine content was reduced by up to 38% in Bna.SCT mutants and 77% in Bna.REF1 mutants, with no observed effects on plant growth or development. These findings suggest that Bna.REF1 is the most effective target for sinapine reduction in transgene-free mutants among the genes tested in our lab.
Authors
- Selvaraju Kanagarajan (ORCID: https://orcid.org/0000-0003-4341-9322)
- L Zhu
- Emelie Ivarson (ORCID: https://orcid.org/0000-0002-7117-7917)
- Eu Sheng Wang (ORCID: https://orcid.org/0000-0002-8499-1221)
- Oliver Moss
- Xueyuan Li
Institutions
- Swedish University of Agricultural Sciences (SE)
Publication Details
- Journal
- Physiologia Plantarum
- Published
- 2026-05-01
- DOI
- https://doi.org/10.1111/ppl.70905
- Primary Topic
- Nitrogen and Sulfur Effects on Brassica
- Type
- article
- Field-Weighted Citation Impact
- 0.00