Tracking the redox reaction of the iron enzyme ribonucleotide reductase using continuous SerialED and SFX
), and re-oxidized states at ∼1.8 Å resolution, capturing three points in a redox reaction. These results demonstrate that c-SerialED can track reversible changes at the redox-site, enabling future time-resolved studies. Comparison between c-SerialED structures and SFX diffraction and emission data confirmed minimal radiation damage. Furthermore, previously reported structures use mercury in the crystallization condition and show mercury-induced conformational changes. Here, we use mercury-free crystallization conditions and reveal a water molecule in the redox center of the reduced state, absent in the previous structures, making these structures more representative of the physiological state.
Authors
- Aaron S. Brewster (ORCID: https://orcid.org/0000-0002-0908-7822)
- Hiroki Makita (ORCID: https://orcid.org/0000-0002-4968-914X)
- Martin Högbom (ORCID: https://orcid.org/0000-0001-5574-9383)
- Oskar Aurelius (ORCID: https://orcid.org/0000-0002-9268-351X)
- Daniel J. Rosenberg (ORCID: https://orcid.org/0000-0001-8017-8156)
- A. Batyuk (ORCID: https://orcid.org/0000-0002-9393-2880)
- Hugo Lebrette (ORCID: https://orcid.org/0000-0002-8081-181X)
- Vittal K. Yachandra (ORCID: https://orcid.org/0000-0002-3983-7858)
- Juliane John (ORCID: https://orcid.org/0000-0001-9626-3670)
- David Moreau (ORCID: https://orcid.org/0000-0002-3193-571X)
- Nicholas K. Sauter (ORCID: https://orcid.org/0000-0003-2786-6552)
- Roberto Alonso‐Mori (ORCID: https://orcid.org/0000-0002-5357-0934)
- Asmit Bhowmick (ORCID: https://orcid.org/0000-0002-6154-6635)
- Daniel W. Paley⧓ (ORCID: https://orcid.org/0000-0003-1161-5142)
- Natalie Minnetian (ORCID: https://orcid.org/0000-0002-1817-184X)
- Jan Kern
- Buster Blomberg
- Xiaodong Zou (ORCID: https://orcid.org/0000-0001-6748-6656)
- Hongyi Xu
- Philipp S. Simon
- Rahul Bannerjee
- Vivek Srinivas
- Kuntal Chatterjee
- Laura Pacoste
- Rohit Kumar
- Junko Yano
- Leland B. Gee
- Gerhard Hofer
Institutions
- Centre National de la Recherche Scientifique (FR)
- Lawrence Berkeley National Laboratory (US)
- Stockholm University (SE)
- TU Dortmund University (DE)
- Science for Life Laboratory (SE)
- SLAC National Accelerator Laboratory (US)
- MAX IV Laboratory (SE)
- Linac Coherent Light Source (US)
- Laboratoire de Microbiologie et Génétique Moléculaires (FR)
- Auburn University (US)
Publication Details
- Journal
- Structure
- Published
- 2026-05-01
- DOI
- https://doi.org/10.1016/j.str.2026.03.006
- Primary Topic
- Metal-Catalyzed Oxygenation Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Foundation for the National Institutes of Health
- National Energy Research Scientific Computing Center
- Knut och Alice Wallenbergs Stiftelse
- Vetenskapsrådet
- National Institute of General Medical Sciences
- SLAC National Accelerator Laboratory