Окиснювальний баланс у фолікулярній рідині яєчників: кореляція з гормональним профілем та відповіддю яєчників у циклах екстракорпорального запліднення

The objective: to evaluate the oxidation-reduction potential (ORP) of follicular fluid (FF) in ovaries as a biomarker of the intrafollicular microenvironment and its association with oocyte maturation and in vitro fertilization (IVF) outcomes.Materials and methods. A retrospective analysis of 212 IVF cycles during 2020–2023 was conducted. ORP in FF was measured using MiOXSYS. The concentrations of follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol, anti-Müllerian hormone and prolactin were measured in blood serum. Correlations between ORP and gonadotropin levels, stimulation parameters, and oocyte outcomes were assessed.Results. The mean ORP value in FF was 86.42 ± 23.23 mV, with wide interindividual variations. Weak but statistically significant positive correlations were observed between ORP and baseline FSH (ρ = 0.166, p = 0.016) and LH levels (ρ = 0.148, p = 0.032). Patients with basal FSH ≥ 8 IU/L exhibited higher ORP levels compared to women with FSH concentration < 8 IU/L (median 93.26 mV vs 81.70 mV, p = 0.041). However, ORP values did not differ significantly between groups stratified by total FSH dose, estradiol levels on chorionic gonadotropin day, or total number of retrieved oocytes. Notably, patients achieving ≥ 12 mature oocytes had a significantly higher oocyte maturation rate (86.19% vs 77.78%, p < 0.001), although their median ORP values were not significantly different (84.17 mV vs 89.68 mV, p = 0.849).Conclusions. ORP in FF reflects subtle endocrine influences but was not directly predictive of oocyte number or maturity. Stable ORP values together with optimal stimulation (≥ 12 mature oocytes) were associated with better oocyte maturation, suggesting ORP as a potential marker of follicular health in IVF.