Validation of MyHPVscore: a high-performance HPV ctDNA lab developed test
> 0.99). Analytes were stable for 96 hours at -20°C and yielded consistent qualitative results after >400 days of storage. No cross-reactivity was observed between hrHPV types or low-risk types (HPV6, HPV11). These results support the use of MyHPVscore as an LDT for detecting HPV+ oropharynx cancer. This methodology establishes an approach and standard controls that can benchmark other emerging ctDNA assays.
Authors
- Collin Brummel (ORCID: https://orcid.org/0000-0001-7198-8560)
- Chaz L. Stucken (ORCID: https://orcid.org/0000-0003-1448-3376)
- Jordan Currie (ORCID: https://orcid.org/0000-0003-1840-6765)
- Keith Casper (ORCID: https://orcid.org/0000-0003-2878-3863)
- Mark E. Prince (ORCID: https://orcid.org/0000-0002-1120-9008)
- Pratyusha Yalamanchi (ORCID: https://orcid.org/0000-0003-0776-3759)
- Andrew G. Shuman (ORCID: https://orcid.org/0000-0002-9305-7860)
- Matthew E. Spector (ORCID: https://orcid.org/0000-0001-7646-6075)
- Muneesh Tewari (ORCID: https://orcid.org/0000-0002-7781-3152)
- J. Chad Brenner (ORCID: https://orcid.org/0000-0003-3238-1111)
- Chandan Bhambhani (ORCID: https://orcid.org/0000-0001-9572-9328)
- R. Akhdar (ORCID: https://orcid.org/0009-0008-7045-8638)
- MD Francis P. Worden
- Molly Heft Neal
- Paul L. Swiecicki
- Michelle L. Mierzwa
- Heather Walline (ORCID: https://orcid.org/0000-0003-3790-5738)
- Colleen G. Hochfelder (ORCID: https://orcid.org/0000-0002-0243-3833)
- Erin Sandford
- Marisa Buchakjian
- Apurva Bhangale (ORCID: https://orcid.org/0000-0001-7262-569X)
- David Forner
- Steven B. Chinn
- Jennifer L. Shah (ORCID: https://orcid.org/0000-0002-3847-8865)
- Kelly M. Malloy
Institutions
- University of Michigan (US)
- Michigan Medicine (US)
- Michigan Center for Translational Pathology (US)
- Michigan Cancer Research Consortium (US)
Publication Details
- Journal
- Journal of Molecular Diagnostics
- Published
- 2026-05-01
- DOI
- https://doi.org/10.1016/j.jmoldx.2026.04.003
- Primary Topic
- Cervical Cancer and HPV Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- American Cancer Society
- National Institute of General Medical Sciences
- National Institute of Dental and Craniofacial Research