Continental-Scale Climatic Zones Drive Reorganization of Lake Sediment Microbiome: Diversity, Assembly and Interaction Networks

Global climate change has altered temperature regimes, hydrological stability, and redox dynamics in inland waters, yet the continental-scale impact of these alterations on sediment microbiomes remains poorly understood. Here, we compiled 562 publicly available 16S rRNA gene datasets from lake sediments across five major climatic zones in China to examine how climatic gradients influence microbial diversity, community assembly, and interaction networks, as well as their associated taxonomic composition and environmental responses. Sediment microbiomes showed clear spatial differentiation in both α- and β-diversity, accompanied by climatic zone-specific taxonomic signatures and biomarker taxa. Community assembly also varied markedly across climatic zones, with stochasticity and dispersal limitation dominating in colder regions, transitional assembly in the south temperate zone, and stronger selective or high-turnover dynamics in the warm subtropics. Importantly, random forest models revealed a clear transition from climate-dominated to anthropogenic-dominated control in sediment microbiome organization: microbial variation in the plateau and temperate regions was primarily associated with climatic and geographic constraints, whereas anthropogenic factors played a more important role in shaping community differentiation in the central subtropical zone. By integrating diversity patterns, taxonomic composition, assembly processes, and network topology, we further propose a three-stage conceptual pattern of sediment microbial community organization along climatic gradients, shifting from a persistence-dominated regime in the cold plateau regions, to an efficiency-dominated regime in the temperate zones, and finally to a plasticity-dominated regime in the warm subtropical regions. These findings would provide a continental-scale framework for understanding sediment microbiome responses to coupled climatic and anthropogenic forcing in inland waters, with implications for future water quality management and ecosystem conservation.