<i>In Vivo</i> Bioimaging and Biodistribution of DiR-Labeled Solid SNEDDS of Black Sticky Rice Extract Using Neusilin® US2 as Solid Carrier

biodistribution and hepatic targeting were investigated in mice using IVIS Spectrum fluorescence imaging (700-900 nm) after oral administration of three formulations: BSRE + corn oil + DiR, blank SNEDDS + DiR, and SNEDDS-BSRE + DiR. Quantitative region-of-interest (ROI) analysis was performed using Living Image® software, and statistical differences were analyzed by one-way ANOVA followed by Tukey's post-hoc test. The S-SNEDDS granules exhibited excellent flow properties (angle of repose = 27°, Carr's index = 13.5%, Hausner's ratio = 1.16) and optimal residual moisture (3.20%), confirming good solid-state stability and reconstitution performance. IVIS imaging revealed formulation-dependent biodistribution profiles: BSRE + corn oil produced diffuse systemic fluorescence, blank SNEDDS + DiR showed weak and transient signals, whereas SNEDDS-BSRE + DiR demonstrated moderate but sustained hepatic fluorescence. Quantitative ROI analysis confirmed significant intergroup differences (p < 0.001). Overall, incorporation of BSRE into the SNEDDS matrix enhanced nanocarrier stability and hepatic localization, highlighting the potential of SNEDDS-BSRE as a bioimaging-guided oral nutraceutical platform for liver-targeted delivery.