μLectures: Ray optics

This presentation is part of the μLecture series about useful microscopy-related topics, organized by the Microscopy Service Centre, IEM CAS. It focuses on the fundamentals of geometrical optics, introducing the basic concepts and principles, including the refractive index and the laws of reflection and refraction of light. Building on these principles, it explains the properties of lenses and demonstrates how they can be applied to understand the function and design of a microscope. It also describes how to correctly set up Köhler illumination, which provides ideal and uniform illumination of the entire microscope field of view.

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Publication Details

Journal
Zenodo (CERN European Organization for Nuclear Research)
Published
2026-04-30
DOI
https://doi.org/10.5281/zenodo.19923471
Primary Topic
Near-Field Optical Microscopy
Type
article
Field-Weighted Citation Impact
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article

μLectures: Ray optics

Barbora Hyklova
Zenodo (CERN European Organization for Nuclear Research)
Near-Field Optical Microscopy
article

μLectures: Ray optics

Barbora Hyklova
article en

Abstract

This presentation is part of the μLecture series about useful microscopy-related topics, organized by the Microscopy Service Centre, IEM CAS. It focuses on the fundamentals of geometrical optics, introducing the basic concepts and principles, including the refractive index and the laws of reflection and refraction of light. Building on these principles, it explains the properties of lenses and demonstrates how they can be applied to understand the function and design of a microscope. It also describes how to correctly set up Köhler illumination, which provides ideal and uniform illumination of the entire microscope field of view.

Zenodo (CERN European Organization for Nuclear Research)
Czech Academy of Sciences, Institute of Experimental Medicine (CZ)
Openalex Percentile: Top 10%
Near-Field Optical Microscopy
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