Saponin Chemistry Controls Anionic Lipid Tolerance and Divalent Metal Ion Responses in Magnetically Alignable Bicelles
, while GA bicelles were disrupted by both ions at low concentrations. Together, these results demonstrate that saponin identity critically determines bicelle compatibility with charged lipids and ionic conditions, establishing design principles for tailoring saponin-based bicelles as versatile, biomimetic alignment media for membrane-protein structural studies.
Authors
- Samuel D. McCalpin (ORCID: https://orcid.org/0000-0003-3196-1237)
- Cletus Obi
- Ayyalusamy Ramamoorthy
Institutions
- Florida State University (US)
- Florida A&M University - Florida State University College of Engineering (US)
- University of Michigan (US)
- National High Magnetic Field Laboratory (US)
Publication Details
- Journal
- bioRxiv (Cold Spring Harbor Laboratory)
- Published
- 2026-04-29
- DOI
- https://doi.org/10.64898/2026.04.25.720808
- Primary Topic
- Lipid Membrane Structure and Behavior
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- National High Magnetic Field Laboratory
- National Institutes of Health
- Division of Materials Research
- High Magnetic Field Laboratory, Chinese Academy of Sciences