Acoustofluidic Biosensors

The rapid and precise detection of biomarkers and pathogens remains a critical challenge in clinical diagnostics. Traditional methodologies are frequently hindered by protracted workflows, complex sample preparation, and reliance on resource-intensive instrumentation. Acoustofluidics—the synergistic integration of acoustics and microfluidics—has emerged as a transformative solution for point-of-care testing (POCT). Bulk acoustic wave (BAW) and surface acoustic wave (SAW) technologies enable the contactless, label-free, and biocompatible manipulation of bioparticles across micro- and nanometer scales. This review critically examines recent advancements in BAW- and SAW-based acoustofluidic biosensors. We elucidate the fundamental principles governing distinct acoustic modes—including Quartz Crystal Microbalance (QCM), film bulk acoustic resonator (FBAR), and Solidly Mounted Resonator (SMR) for BAW and Rayleigh and Love waves for SAW—and evaluate their specific roles in liquid-phase sensing, particle sorting, and cellular focusing. Results show that integrating on-chip sample preparation accelerates diagnostic workflows, reducing assay times to under 10 min. Coupling acoustic manipulation with optical, mass-based, or electrochemical modalities effectively overcomes fundamental diffusion limits, achieving ultrasensitive, multimodal detection. We address translational challenges—acoustothermal heating, biofouling, and scalable integration. Following a discussion of clinical applications in oncology and infectious diseases, we map emerging trajectories, emphasizing AI-driven intelligent microfluidics, modular architectures, and flexible wearable platforms that will ultimately democratize continuous precision diagnostics.